Stability issues of RT‐PCR testing of SARS‐CoV‐2 for hospitalized patients clinically diagnosed with COVID‐19
False negative results from the standard test for COVID-19 mean that more people probably have the virus than might be expected. This would mean that a smaller proportion of the total is dying and COVID-19 might be less lethal than it seems
610 hospitalized patients from Wuhan studied between February 2, 2020, and February 17, 2020.
The study revealed a potentially high false negative rate of real-time reverse-transcriptase polymerase chain reaction (RT-PCR) testing for SARS-CoV-2 in the 610 hospitalized patients clinically diagnosed with COVID-19 during the 2019 outbreak.
RT-PCR results from several tests at different points were variable from the same patients during the course of diagnosis and treatment of these patients.
These results suggested the urgent needs for the standard of procedures of sampling from different anatomic sites, sample transportation, optimization of RT-PCR, serology diagnosis/screening for SARS-CoV-2 infection, and distinct diagnosis from other respiratory diseases such as fluenza infections as well.